sequence 3 Search Results


fndc5 specific sirna (sense sequence: 5′gauggccucuaagaacaaa3′; antisense sequence: 3′cuaccggagauucuuguuu5′  (Ribobio co)
90
Ribobio co fndc5 specific sirna (sense sequence: 5′gauggccucuaagaacaaa3′; antisense sequence: 3′cuaccggagauucuuguuu5′
<t>FNDC5</t> deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf - α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 3
Fndc5 Specific Sirna (Sense Sequence: 5′Gauggccucuaagaacaaa3′; Antisense Sequence: 3′Cuaccggagauucuuguuu5′, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/fndc5+specific+sirna++sense+sequence++5+gauggccucuaagaacaaa3+++antisense+sequence++3+cuaccggagauucuuguuu5+/pmc06444535-82-1-13
Average 90 stars, based on 1 article reviews
fndc5 specific sirna (sense sequence: 5′gauggccucuaagaacaaa3′; antisense sequence: 3′cuaccggagauucuuguuu5′ - by Bioz Stars, 2026-09
90/100 stars
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sequence 3  (Chemie GmbH)
90
Chemie GmbH sequence 3
<t>FNDC5</t> deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf - α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 3
Sequence 3, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/sequence+3/pm36625790-35-12-1
Average 90 stars, based on 1 article reviews
sequence 3 - by Bioz Stars, 2026-09
90/100 stars
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random sequence 3'utr  (SwitchGear Genomics)
90
SwitchGear Genomics random sequence 3'utr
<t>FNDC5</t> deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf - α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 3
Random Sequence 3'utr, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/the+random+sequence+3+utr/10__1161_slash_jaha__120__019862-129-86-38
Average 90 stars, based on 1 article reviews
random sequence 3'utr - by Bioz Stars, 2026-09
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t2*-weighted echo-planar imaging sequence 3 t siemens magnetom verio whole-body mr unit  (Siemens AG)
90
Siemens AG t2*-weighted echo-planar imaging sequence 3 t siemens magnetom verio whole-body mr unit
<t>FNDC5</t> deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf - α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 3
T2* Weighted Echo Planar Imaging Sequence 3 T Siemens Magnetom Verio Whole Body Mr Unit, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/t2++weighted+echo+planar+imaging+sequence+3+t+siemens+magnetom+verio+whole+body+mr+unit/pmc09433840-75-10-16
Average 90 stars, based on 1 article reviews
t2*-weighted echo-planar imaging sequence 3 t siemens magnetom verio whole-body mr unit - by Bioz Stars, 2026-09
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mitarget mirna target sequence (3′utr) expression clones for ikk α  (Labomics Inc)
90
Labomics Inc mitarget mirna target sequence (3′utr) expression clones for ikk α
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Mitarget Mirna Target Sequence (3′Utr) Expression Clones For Ikk α, supplied by Labomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/mitarget+mirna+target+sequence++3+utr++expression+clones+for+ikk+%CE%B1/pmc04738320-135-18-22
Average 90 stars, based on 1 article reviews
mitarget mirna target sequence (3′utr) expression clones for ikk α - by Bioz Stars, 2026-09
90/100 stars
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responsive sequence 3  (InsuLine Medical Ltd)
90
InsuLine Medical Ltd responsive sequence 3
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Responsive Sequence 3, supplied by InsuLine Medical Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/responsive+sequence+3/pm19713685-90-8-10
Average 90 stars, based on 1 article reviews
responsive sequence 3 - by Bioz Stars, 2026-09
90/100 stars
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mmre sequence 3 t siemens trio mr-system  (Siemens AG)
90
Siemens AG mmre sequence 3 t siemens trio mr-system
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Mmre Sequence 3 T Siemens Trio Mr System, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/mmre+sequence+3+t+siemens+trio+mr+system/pmc05842309-89-10-14
Average 90 stars, based on 1 article reviews
mmre sequence 3 t siemens trio mr-system - by Bioz Stars, 2026-09
90/100 stars
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mprage sequence 3 t siemens prisma  (Siemens AG)
90
Siemens AG mprage sequence 3 t siemens prisma
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Mprage Sequence 3 T Siemens Prisma, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/mprage+sequence+3+t+siemens+prisma/pmc09338196-52-7-11
Average 90 stars, based on 1 article reviews
mprage sequence 3 t siemens prisma - by Bioz Stars, 2026-09
90/100 stars
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par-1 antagonist peptide (sequence 3-mpr-fcha-cha-rkpndk-nh2)  (KU Leuven)
90
KU Leuven par-1 antagonist peptide (sequence 3-mpr-fcha-cha-rkpndk-nh2)
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Par 1 Antagonist Peptide (Sequence 3 Mpr Fcha Cha Rkpndk Nh2), supplied by KU Leuven, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/par+1+antagonist+peptide++sequence+3+mpr+fcha+cha+rkpndk+nh2+/pm17197525-55-1-14
Average 90 stars, based on 1 article reviews
par-1 antagonist peptide (sequence 3-mpr-fcha-cha-rkpndk-nh2) - by Bioz Stars, 2026-09
90/100 stars
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axial spin-echo proton density sequence 3 t siemens skyra whole body mr unit  (Siemens AG)
90
Siemens AG axial spin-echo proton density sequence 3 t siemens skyra whole body mr unit
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Axial Spin Echo Proton Density Sequence 3 T Siemens Skyra Whole Body Mr Unit, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/axial+spin+echo+proton+density+sequence+3+t+siemens+skyra+whole+body+mr+unit/pm36307532-58-21-27
Average 90 stars, based on 1 article reviews
axial spin-echo proton density sequence 3 t siemens skyra whole body mr unit - by Bioz Stars, 2026-09
90/100 stars
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bfgf probe corresponding to the first 530 nucleotides of sequence 3' to the translational stop codon  (Promega)
90
Promega bfgf probe corresponding to the first 530 nucleotides of sequence 3' to the translational stop codon
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Bfgf Probe Corresponding To The First 530 Nucleotides Of Sequence 3' To The Translational Stop Codon, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/bfgf+probe+corresponding+to+the+first+530+nucleotides+of+sequence+3++to+the+translational+stop+codon/10__1007_slash_bf02631248-91-1-47
Average 90 stars, based on 1 article reviews
bfgf probe corresponding to the first 530 nucleotides of sequence 3' to the translational stop codon - by Bioz Stars, 2026-09
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g oligo refers to the following sequence: 3′ g-a-t-g-a-a-g-t-g-t-g-a-t-g-t-a-g-a-a-g-a-t-g-t-g 5′  (MWG-Biotech ag)
90
MWG-Biotech ag g oligo refers to the following sequence: 3′ g-a-t-g-a-a-g-t-g-t-g-a-t-g-t-a-g-a-a-g-a-t-g-t-g 5′
( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), <t>and</t> <t>transfected</t> with the wild-type ikk α- or the <t>arnt</t> 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
G Oligo Refers To The Following Sequence: 3′ G A T G A A G T G T G A T G T A G A A G A T G T G 5′, supplied by MWG-Biotech ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+3/g+oligo+refers+to+the+following+sequence++3++g+a+t+g+a+a+g+t+g+t+g+a+t+g+t+a+g+a+a+g+a+t+g+t+g+5+/10__1021_slash_ja0348795-80-6-4
Average 90 stars, based on 1 article reviews
g oligo refers to the following sequence: 3′ g-a-t-g-a-a-g-t-g-t-g-a-t-g-t-a-g-a-a-g-a-t-g-t-g 5′ - by Bioz Stars, 2026-09
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Image Search Results


FNDC5 deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf - α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 3

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: FNDC5 deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf - α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 3

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques: Western Blot, Activation Assay

FNDC5 deficiency enhanced HFD-induced oxidative stress and phosphorylated JAK2 and STAT3 level in heart. a Superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in heart. b Expression of NAPDH oxidases (NOX2 and NOX4) protein in heart. c Phosphorylated JAK2 and STAT3 level in heart. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 6

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: FNDC5 deficiency enhanced HFD-induced oxidative stress and phosphorylated JAK2 and STAT3 level in heart. a Superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in heart. b Expression of NAPDH oxidases (NOX2 and NOX4) protein in heart. c Phosphorylated JAK2 and STAT3 level in heart. Values are mean ± SEM. *P < 0.05 vs. WT. † P < 0.05 vs. Ctrl. n = 6

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques: Activity Assay, Expressing

FNDC5 deficiency enhanced palmitate-induced inflammation and NOX4 expression in primary cardiomyocytes (CMs). a Effects of palmitate (PA, 400 μM) treatment on Tnf - α, Il1b and Il6 mRNA levels in CMs of WT and FNDC5 −/− mice. b Effects of PA treatment on NOX2 and NOX4 protein level in CMs of WT and FNDC5 −/− mice. The measurement was 24 h after PA treatment for measuring the mRNA levels or protein levels. PA: Palmitate; Veh: Vehicle; F −/− : FNDC5 −/− . Values are mean ± SEM. *P < 0.05 vs. Veh. † P < 0.05 vs. CMs-WT. n = 3

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: FNDC5 deficiency enhanced palmitate-induced inflammation and NOX4 expression in primary cardiomyocytes (CMs). a Effects of palmitate (PA, 400 μM) treatment on Tnf - α, Il1b and Il6 mRNA levels in CMs of WT and FNDC5 −/− mice. b Effects of PA treatment on NOX2 and NOX4 protein level in CMs of WT and FNDC5 −/− mice. The measurement was 24 h after PA treatment for measuring the mRNA levels or protein levels. PA: Palmitate; Veh: Vehicle; F −/− : FNDC5 −/− . Values are mean ± SEM. *P < 0.05 vs. Veh. † P < 0.05 vs. CMs-WT. n = 3

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques: Expressing

Exogenous FNDC5 pretreatment attenuated palmitate-induced inflammation and oxidative stress in CMs. a , b Effects of exogenous FNDC5 pretreatment on cardiac hypertrophy markers ( Nppa, Nppb and Myh7 ) and inflammation markers ( Tnf - α, Il1b and Il6 ) mRNA levels in PA-stimulated CMs. c Effects of exogenous FNDC5 pretreatment on NO production in cell culture supernatant of PA-stimulated CMs. d Effects of exogenous FNDC5 pretreatment on NOX expression in PA-stimulated CMs. PA: Palmitate; Veh: Vehicle. Values are mean ± SEM. *P < 0.05 vs. Veh. † P < 0.05 vs. PA. n = 6

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: Exogenous FNDC5 pretreatment attenuated palmitate-induced inflammation and oxidative stress in CMs. a , b Effects of exogenous FNDC5 pretreatment on cardiac hypertrophy markers ( Nppa, Nppb and Myh7 ) and inflammation markers ( Tnf - α, Il1b and Il6 ) mRNA levels in PA-stimulated CMs. c Effects of exogenous FNDC5 pretreatment on NO production in cell culture supernatant of PA-stimulated CMs. d Effects of exogenous FNDC5 pretreatment on NOX expression in PA-stimulated CMs. PA: Palmitate; Veh: Vehicle. Values are mean ± SEM. *P < 0.05 vs. Veh. † P < 0.05 vs. PA. n = 6

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques: Cell Culture, Expressing

FNDC5 overexpression (OE) attenuated cardiac hypertrophy, inflammation and oxidative stress in HFD-fed mice. a , b Serum and heart/muscle FNDC5 levels after lentiviral vector-mediated FNDC5 overexpression. c Cardiomyocyte area in heart determined by H&E staining. d , e mRNA levels of the cardiac hypertrophy markers ( Nppa, Nppb and Myh7 ) and inflammation markers ( Tnf - α, Il1b and Il6 ). f SOD activity and MDA level in heart. g NOX2, NOX4 level and NFκB inactivation in heart. h Phosphorylation levels of p38 and ERK in heart. Values are mean ± SEM. *P < 0.05 vs. vector. n = 6

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: FNDC5 overexpression (OE) attenuated cardiac hypertrophy, inflammation and oxidative stress in HFD-fed mice. a , b Serum and heart/muscle FNDC5 levels after lentiviral vector-mediated FNDC5 overexpression. c Cardiomyocyte area in heart determined by H&E staining. d , e mRNA levels of the cardiac hypertrophy markers ( Nppa, Nppb and Myh7 ) and inflammation markers ( Tnf - α, Il1b and Il6 ). f SOD activity and MDA level in heart. g NOX2, NOX4 level and NFκB inactivation in heart. h Phosphorylation levels of p38 and ERK in heart. Values are mean ± SEM. *P < 0.05 vs. vector. n = 6

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques: Over Expression, Plasmid Preparation, Staining, Activity Assay

Schematic illustrates a possible mechanism of FNDC5 in obesity-induced cardiac hypertrophy. Obesity-induced lipid overload has a great impact on the production of reactive oxygen species (ROS) and upregulation of TNF-α, IL-1β and IL-6 levels, which leads to cardiac inflammation and oxidative stress. The enhanced cardiac inflammation and oxidative stress are involved in pathogenesis of cardiac injury and remodeling. FNDC5 may exert its protective function on the enhanced inflammation and oxidative stress via JAK2/STAT3 pathway through an unknown receptor

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: Schematic illustrates a possible mechanism of FNDC5 in obesity-induced cardiac hypertrophy. Obesity-induced lipid overload has a great impact on the production of reactive oxygen species (ROS) and upregulation of TNF-α, IL-1β and IL-6 levels, which leads to cardiac inflammation and oxidative stress. The enhanced cardiac inflammation and oxidative stress are involved in pathogenesis of cardiac injury and remodeling. FNDC5 may exert its protective function on the enhanced inflammation and oxidative stress via JAK2/STAT3 pathway through an unknown receptor

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques:

Echocardiographic assessment of left ventricle functions in mice

Journal: Journal of Translational Medicine

Article Title: FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress

doi: 10.1186/s12967-019-1857-8

Figure Lengend Snippet: Echocardiographic assessment of left ventricle functions in mice

Article Snippet: The FNDC5 specific siRNA (sense sequence: 5′GAUGGCCUCUAAGAACAAA3′; antisense sequence: 3′CUACCGGAGAUUCUUGUUU5′) was purchased from RiboBio (Guangzhou, China).

Techniques:

( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), and transfected with the wild-type ikk α- or the arnt 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.

Journal: Scientific Reports

Article Title: Notch-regulated miR-223 targets the aryl hydrocarbon receptor pathway and increases cytokine production in macrophages from rheumatoid arthritis patients

doi: 10.1038/srep20223

Figure Lengend Snippet: ( a ) Ingenuity analysis of putative miR-223 targets as determined in silico . Signalling pathways with the highest scores are indicated. ( b ) Luciferase reporter assays of HEK-293 cells transduced with pre-miR-223 or a control (mock), and transfected with the wild-type ikk α- or the arnt 3′UTR reporter constructs. Luciferase activity was normalised to that of mock-transfected cells. Data shown as mean ± SEM of triplicates in one representative experiment ( n = 3). ( c ) Immunoblot analysis of extracts of HEK-293 cells expressing control or pre-miR-223, probed with anti-ARNT and -hnRNP U (nuclear loading control); the hnRNP U/ARNT ratio in mock-transfected cells is equal to 1. For ( b , c ), data are representative of at least three experiments. ( d ) CYP1A1 mRNA levels in mock and pre-miR-223-expressing THP-1 cells treated with B a P or vehicle. Data shown as mean ± SEM of RQ from triplicates in three independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.

Article Snippet: Cells were selected with puromycin (3 μg/ml) and transfected with miTarget miRNA Target Sequence (3′UTR) Expression Clones for arnt and ikk α (Labomics).

Techniques: In Silico, Luciferase, Transduction, Control, Transfection, Construct, Activity Assay, Western Blot, Expressing, MANN-WHITNEY